We are able to offer custom gene cloning as well as expression and stable cell-line development services. Using your desired vector and clonal selection methods, we are able to isolate and express the desired gene for you in addition to establishing clonal cell-lines with the highest levels of target gene expression. This service includes:
- Isolation of gene interest
- Generation of recombinant construction
- Verification of recombinant construct by restriction mapping and sequence analysis
- Preparation of plasmid contruct DNA
- Introduction of plasmid DNA to mammalian cells
- Selection of over-expressing cells
- Small scale expression and purification study assessed by SDS-PAGE and Western Blot
- Estimation of protein yield
- Development of stable cell-line and expression of chosen gene interest
With our expression and plasmid design and synthesis, we are able to design various plasmids simultaneously with our in house LIC vector system. With this system we have the potential to create numerous plasmids that contain a variety of purification tags, cleavage sites, and mutations. We always strive to provide quality plasmids for our clients in μg quantities. Please contact us for more information and/or price quotes.
