In our lab located in San Diego California, we are able to clone an insert of interest into a specific vector that has been provided by you. Upon completion, you will receive 2-3µg of DNA, sequence verification, as well as visualized results from gel electrophoresis.This service includes:TransformationLigationPrimer Design & SynthesisDNA SequencingMini-prep (Maxi-prep is available at
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We are able to offer custom gene cloning as well as expression and stable cell-line development services. Using your desired vector and clonal selection methods, we are able to isolate and express the desired gene for you in addition to establishing clonal cell-lines with the highest levels of target gene expression. This service includes: Isolation
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With over 15 years of experience in E.Coli Expressions, we have the expertise to efficiently enhance protein expression by testing over 96 different conditions here in house. Our turnaround time is fast and our results are reproducible. This service includes: Transformation of plasmids into various E.Coli expression strains Evaluation of expression by SDS-PAGE or Western
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Our company has over 15 years of Baulovirus Protein Expression. From plasmid to baculovirus production, we offer a quick and versatile option for all of your expression needs. This service includes: Transfection of insect cells and generation of recombinant virus Viral production and plaque purification Test expression of recombinant virus clones Amplification and purification of
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Over the years, Protein Purification has become our most elite service that we have to offer. We have the capabilities to extract and purify proteins from various sources such as bacteria, yeast, insect, mammalian cells, in addition to tissue sources. We perform purifications for a variety of different needs from high throughput screens to crystallography
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We offer protein production in Mammalian cell lines such as HEK293 and CHO both transiently and stable. This service includes: Expression optimization in various cell lines High expression systems for transient expression with 1mg/mL Yield Fast stable pool and stable clone generation Large scale protein production in shake flasks or Wave machine Cell harvesting, clarification
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